[Todos] Seminario en el DF - Peter Hinterdorfer (JKU): VIERNES 19/6, 14hs, Aula Seminario, Pab. I

[Augusto Roncaglia]

SEMINARIO EN EL DEPARTAMENTO DE F�SICA FCEYN - UBA


                        En el Aula Seminario, 2do piso, Pab. I,

                        Viernes 19/6, 14hs:

                        PETER HINTERDORFER

                        Johannes Kepler University Linz.


*                        Antibodies on the Video Screen: Bipedal Antibody
Walking on Membranes observed with High-Speed AFM*

Binding of antibodies to their cognate antigens is fundamental for adaptive
immunity. Molecular engineering of antibodies for therapeutic and
diagnostic purposes emerges to be one of the major technologies in
combating many human diseases. Despite its importance, a detailed
description of the nanomechanical process of antibody-antigen binding and
dissociation on the molecular level is lacking. Here, we utilize single
molecule force spectroscopy and high-speed atomic force microscopy to
examine the dynamics of antibody recognition and uncover a new principle.
Contradicting the current textbook view, antibodies do not remain
stationary on surfaces of regularly spaced epitopes; they rather exhibit
“bipedal� random walking. As monovalent Fabs do not move, steric strain is
identified as the origin of short-lived bivalent binding. Randomly walking
antibodies gather in transient clusters that might serve as docking sites
for the complement system and/or phagocytes. Our findings will inspire the
rational design of antibodies and multivalent receptors to exploit/inhibit
steric strain-induced dynamic effects.
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