[Todos] Seminario en el DF - Wolfgang Fritzsche (IPHT): VIERNES 18/9, 14hs, Aula Seminario, Pab. I

[Augusto Roncaglia]

SEMINARIO EN EL DEPARTAMENTO DE F�SICA FCEYN - UBA


                        En el Aula Seminario, 2do piso, Pab. I,

                        Viernes 18/9, 14hs:

                        WOLFGANG FRITZSCHE

                        Leibniz Institute of Photonic Technology



*                        Bioanalytics using single plasmonic nanostructures*

Novel requirements for bioanalytical methods emerge due to trends such as
personalized medicine or pathogen detection in environment and food. Here,
novel tools for diagnostics are needed, to be used outside of dedicated
laboratories and with less qualified personnel, and with minimal costs.
Plasmonic nanostructures promise to provide sensing capabilities with the
potential for ultrasensitive and robust assays in a high parallelization.
Upon binding of molecules, the localized surface plasmon resonance (LSPR)
of these structure is changed, and can be used as sensoric readout. Here
the use of individual nanostructures (such as gold nanoparticles) for the
detection and manipulation of biomolecules (e.g. DNA) based on optical
approaches is presented.

Holes in a Cr layer present an interesting approach for bioanalytics. They
are used to detect even single plasmonic nanoparticles as labels or to
sense the binding of DNA on these particles. This hybrid system of hole and
particle allows for simple (just using RGB-signals of a CCD) but a highly
sensitive (one nanoparticle sensitivity) detection. Moreover, the binding
of a molecular layer around the particles can be detected using
spectroscopic features of just an individual particle.

The change in LSPR of individual metal nanoparticles is utilized to monitor
the binding of DNA directly or via DNA-DNA interaction. The influence of
different size (length) as well as position (distance to the particle
surface) is thereby studied using a dark-field approach developed a century
ago. The established serial approach is now further developed into a
parallel readout using imaging spectrometric sensing based on
interferometry and Fourier transformation.
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