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<h1><b><span lang=ES-TRAD style='font-family:"Calibri","sans-serif";text-decoration:
none'>Coloquio<o:p></o:p></span></b></h1>
<p class=MsoNormal><span lang=ES-TRAD style='font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal style='text-autospace:none'><b><span lang=ES
style='font-family:"Calibri","sans-serif"'>INFIP</span></b><span lang=ES
style='font-family:"Calibri","sans-serif"'>-INSTITUTO DE FÍSICA DEL PLASMA<o:p></o:p></span></p>
<p class=MsoNormal style='text-autospace:none'><span lang=ES-TRAD
style='font-family:"Calibri","sans-serif"'>Depto. de Física, FCEN, UBA -
CONICET<o:p></o:p></span></p>
<p class=MsoNormal><span lang=ES-TRAD style='font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal><u><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Título<o:p></o:p></span></u></p>
<p class=MsoNormal><u><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'><o:p><span
style='text-decoration:none'> </span></o:p></span></u></p>
<p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Nanoscale
Bioelectric Plasma and Membrane Permeabilization<o:p></o:p></span></b></p>
<p class=MsoNormal><u><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'><o:p><span
style='text-decoration:none'> </span></o:p></span></u></p>
<p class=MsoNormal><u><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Disertante<o:p></o:p></span></u></p>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>P.
Thomas Vernier<o:p></o:p></span></b></p>
<p class=MsoNormal><span style='font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Calibri","sans-serif";
color:#444444;background:white'>Frank Reidy Research Center for Bioelectrics,
Old Dominion University, Norfolk, VA 23508, USA<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal><u><span lang=ES style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Fecha<o:p></o:p></span></u></p>
<p class=MsoNormal style='text-autospace:none'><span lang=ES style='font-size:
10.0pt;font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal style='text-autospace:none'><b><span lang=ES
style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Martes 31 de mayo a
las 14 hs.<o:p></o:p></span></b></p>
<p class=MsoNormal style='text-autospace:none'><b><span lang=ES
style='font-size:10.0pt;font-family:"Calibri","sans-serif"'><o:p> </o:p></span></b></p>
<h1><span lang=ES style='font-family:"Calibri","sans-serif"'>Lugar<o:p></o:p></span></h1>
<p class=MsoNormal><span lang=ES style='font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal><b><span lang=ES-TRAD style='font-size:11.0pt;font-family:
"Calibri","sans-serif"'>Aula Seminario, Depto. de Física, 2<sup>do</sup> piso,
Pab. </span></b><b><span style='font-size:11.0pt;font-family:"Calibri","sans-serif"'>I<o:p></o:p></span></b></p>
<p class=MsoNormal><span style='font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<h1 style='text-autospace:ideograph-other'><span style='font-family:"Calibri","sans-serif"'>Resumen<o:p></o:p></span></h1>
<p class=MsoNormal><span style='font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal style='text-align:justify'><span style='font-size:10.0pt;
font-family:"Calibri","sans-serif";color:#444444;background:white'>Pulsed electric
fields of sufficient intensity produce long-lived permeabilizing structures in
biological membranes. Transport of normally impermeant substances across
electroporated membranes can continue for several minutes, but cells recover
when the electrical energy dose is properly calibrated and delivered.
Electroporation (electropermeabilization) technology is used for delivery of
genetic and pharmacological material into cells and tissues and for other
nonmechanical processing of bio-matter in the laboratory, the clinic, and the
industrial plant. Despite decades of study the detailed mechanisms of
electroporation and the network of processes and structures associated with the
persistent permeabilized state remain resistant to characterization.<o:p></o:p></span></p>
<p class=MsoNormal style='text-align:justify'><span style='font-size:10.0pt;
font-family:"Calibri","sans-serif";color:#444444;background:white'>One proposed
mechanism associates persistent membrane permeabilization following
electroporation with the peroxidation of membrane lipids. Membranes containing
oxidized lipid products are leaky, and they remain so until repaired. Cellular
levels of reactive oxygen species are known to increase after pulsed electric
field exposure, and peroxidation products have been detected in lipid bilayers
and cell membranes after electropermeabilization.<o:p></o:p></span></p>
<p class=MsoNormal style='text-align:justify'><span style='font-size:10.0pt;
font-family:"Calibri","sans-serif";color:#444444;background:white'>We present a
hypothetical scheme for the generation of hydroxyl radicals in the interior of
the lipid bilayer when an electric field is applied, and for the immediate
reaction of these radicals with the phospholipid hydrocarbon tails located less
than 1 nm away. Evidence from experiment and from molecular simulations
supports the plausibility of this proposed mechanism.<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p>
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