<div dir="ltr"><div><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">Hola,</span></p><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><br></span></p><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">Queríamos anunciar la realización del <span class="gmail-il">curso</span> de postgrado "<span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il"><span class="gmail-il">MICROSCOPÍA</span></span> </span><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"></span><span class="gmail-m_-6687019147849365094gmail-il">ÓPTICA</span> <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">Y</span></span> DE
<span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">FLUORESCENCIA</span></span> <span class="gmail-m_-6687019147849365094gmail-il">1</span>" (puntaje propuesto para doctorado
en la <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"></span>FCEyN: 3 puntos)<br></span></p><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-il"><br></span></span></p><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-il">El curso</span> está dirigido a estudiantes de
postgrado en las áreas de Biología, Química, Física, Medicina, Farmacia, Bioquímica <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">y</span></span> Biotecnología.</span></p><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><br></span></p><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">El objetivo general será transmitir a
los alumnos los conocimientos básicos de <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"></span>técnicas de <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il"><span class="gmail-il">microscopía</span></span></span> <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">óptica</span> <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">y</span></span> de <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">fluorescencia</span></span>
<span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">y</span></span> sus aplicaciones en estos campos de la ciencia;
<span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"> se detalla el programa y bibliografía al final de este mail</span>.</span><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"> </span>Dicho <span class="gmail-il">curso</span> se dictará conjuntamente entre los
Dptos. de Química Biológica <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">y</span></span> Física; los
profesores responsables son Hernán Grecco (Dpto. de <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"> </span>Física) <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">y</span></span> Valeria <span class="gmail-il">Levi</span>
(Qca. Biológica).</span><br class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"></p><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"></span><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">
<br class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">
</span></p>
<p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">El <span class="gmail-il">curso</span> se
desarrollará entre el 4 <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641m_8437409081670997045gmail-il gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-il">y</span></span> 18 de junio de 2018 de 9 a 17
hs. </span></p>
<p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">Los interesados podrán inscribirse a
través del sistema Guaraní <span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"> </span>(<a href="http://inscripciones.exactas.uba.ar/exactas/" target="_blank">http://inscripciones.exactas.uba.ar/exactas/</a></span><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg" lang="EN-US"><a href="http://uba.ar/" class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg" target="_blank"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg" lang="ES-AR"></span></a></span><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">) </span><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg" lang="EN-US"><a href="https://goo.gl/forms/quoYjm0zYyzNNQC82" class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg" target="_blank"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg" lang="ES-AR"></span></a></span><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"></span></p>
<p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"> </span></p><p class="MsoNormal gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg"><span class="gmail-m_-6687019147849365094gmail-m_6011002015526019351gmail-m_-7462266695709091641gmail_msg">Agradecemos la difusión</span><br></p></div><div> <br>----<br></div><br><div>
<span name="date">
<p class="MsoNormal" style="text-align:justify;margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><u><span style="font-size:12pt" lang="ES-TRAD">PROGRAMA
ANALÍTICO Y BIBLIOGRAFÍA DEL <span class="gmail-il">CURSO</span>:<span></span></span></u></b></p>
<p class="MsoNormal" style="text-align:justify;margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><u><span style="font-size:12pt" lang="ES-TRAD"><span><span style="text-decoration:none"><br></span></span></span></u></b></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><span style="text-transform:uppercase" lang="ES-TRAD">Módulo 1: Fundamentos<span></span></span></b></p>
<p class="MsoNormal" style="text-align:justify;margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><span style="font-size:11pt" lang="ES-TRAD">1.1 - Fotoquímica y
Fluorescencia<span></span></span></b></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="ES-TRAD">Espectroscopía de absorción molecular. </span><span style="font-size:11pt"><span> </span>Características de la emisión de
fluorescencia: espectros de excitación y emisión; corrimiento de Stokes.
Desactivación de la fluorescencia y transferencia de energía (mecanismo dipolar
- Förster). Anisotropía de emisión. Aplicaciones de estas técnicas al estudio
de sistemas biológicos<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Fluoróforos y sondas de
fluorescencia comunes para el estudio de sistemas biológicos.<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Estrategias de marcación con
sondas fluorescentes: Sondas intrínsecas y extrínsecas; técnicas básicas de
bioconjugación; inmunomarcación.<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><u><span style="font-size:11pt"><span><span style="text-decoration:none"> </span></span></span></u></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt"><span> </span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><span style="font-size:11pt">1.2 – <span class="gmail-il">Microscopía</span> óptica<span></span></span></b></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Principios básicos de óptica. Descripción
básica del microscopio por óptica geométrica: trazado de rayos; formación de
imágenes. Interferencia y difracción. Leyes de Snell y reflexión total.
Interferencia y difracción. Magnificación y resolución. Distancia de trabajo.<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Componentes básicos de un
microscopio óptico: fuentes de iluminación coherentes e incoherentes; objetivo
y otras lentes (ocular, <i>tube lens</i>,
condensador, etc.); detectores (puntuales, cámaras).<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt"><span class="gmail-il">Microscopía</span> de campo claro:
principios básicos, iluminación de Koehler. Microscopios derechos e invertidos<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Técnicas de generación de
contraste: campo oscuro, contraste de fase, DIC (differential interference
contrast)<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt"><span> </span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><span style="font-size:11pt">1.3 – <span class="gmail-il">Microscopía</span> de
fluorescencia de campo amplio<span></span></span></b></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Componentes básicos del
microscopio de fluorescencia de campo amplio de transmisión y epiiluminación.<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Adquisición de imágenes por
cámaras CCD<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Análisis cuantitativo de
imágenes<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;text-align:justify;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">Aplicaciones<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><span style="font-size:11pt;text-transform:uppercase"><span> </span></span></b></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><span style="font-size:11pt">1.4-– <span class="gmail-il">Microscopía</span> de
fluorescencia: técnicas <span></span></span></b></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">2.1.1 – <span class="gmail-il">Microscopía</span> confocal<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">2.1.2 – <span class="gmail-il">Microscopía</span> de excitación por dos fotones<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="EN-US">2.1.3 – <span class="gmail-il">Microscopía</span> TIRF (<i>total internal reflection -fluorescence-
microscopy</i>)<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt 21.3pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt">2.1.3 – <span class="gmail-il">Microscopía</span> confocal con disco giratorio.<span></span></span></p>
<p class="MsoNormal" style="margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><u><span style="font-size:11pt"><span><span style="text-decoration:none"> </span></span></span></u></p>
<p class="MsoNormal" style="text-align:justify;margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><u><span style="font-size:12pt"><span><span style="text-decoration:none"> </span></span></span></u></b></p>
<p class="MsoNormal" style="text-align:justify;margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><u><span style="font-size:12pt" lang="ES-TRAD">Bibliografía
general:<span></span></span></u></b></p>
<p class="MsoNormal" style="text-align:justify;margin:0cm 0cm 0.0001pt;font-size:10pt;font-family:"Times New Roman","serif""><b><u><span style="font-size:12pt" lang="ES-TRAD"><span><span style="text-decoration:none"> </span></span></span></u></b></p>
<ol style="margin-top:0cm;margin-bottom:0cm" start="1" type="1">
<li class="MsoNormal" style="margin:0cm 0cm 6pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="EN-US">JR
Lakowicz. Principles of fluorescence spectroscopy.<span> </span></span><span style="font-size:11pt" lang="IT">Springer; 2 edition (June 30, 1999)</span><span style="font-size:11pt" lang="ES-TRAD"><span></span></span></li>
<li class="MsoNormal" style="margin:0cm 0cm 6pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="EN-US">B
Valeur. Molecular fluorescence: Principles and applications. </span><span style="font-size:11pt" lang="IT">Wiley-VCH; 1 edition
(October 11, 2001)</span><span style="font-size:11pt" lang="ES-TRAD"><span></span></span></li>
<li class="MsoNormal" style="margin:0cm 0cm 6pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="EN-US">Digital
microscopy. Volume 72, Second Edition: A second edition of "Video
Microscopy" (Methods in Cell Biology). </span><span style="font-size:11pt" lang="ES-TRAD">Academic Press; 2 edition (<span>December 19, 2003</span>)<span></span></span></li>
<li class="MsoNormal" style="margin:0cm 0cm 6pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="EN-US">Hecht
E. Optics. Addison Wesley; 4 edition (August 2, 2001)<span></span></span></li>
<li class="MsoNormal" style="margin:0cm 0cm 6pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="EN-US">DB
Murphy. Fundamental of Light microscopy and electronic imaging. John Wiley
& Son, Inc. (2001)<span></span></span></li>
<li class="MsoNormal" style="margin:0cm 0cm 6pt;font-size:10pt;font-family:"Times New Roman","serif""><span style="font-size:11pt" lang="EN-US">R
Wayne. Light and Video Microscopy. Academic Press (2009)</span></li></ol></span></div><br></div>