[Todos] Coloquio

[Diana Grondona]

Coloquio


 

INFIP-INSTITUTO DE FÍSICA DEL PLASMA

Depto. de Física, FCEN, UBA - CONICET

 

Título

 

Nanoscale Bioelectric Plasma and Membrane Permeabilization

 

Disertante

 

P. Thomas Vernier

 

Frank Reidy Research Center for Bioelectrics, Old Dominion University,
Norfolk, VA 23508, USA

 

Fecha

 

Martes 31 de mayo a las 14 hs.

 


Lugar


 

Aula Seminario, Depto. de Física, 2do piso, Pab. I

 


Resumen


 

Pulsed electric fields of sufficient intensity produce long-lived
permeabilizing structures in biological membranes. Transport of normally
impermeant substances across electroporated membranes can continue for
several minutes, but cells recover when the electrical energy dose is
properly calibrated and delivered. Electroporation (electropermeabilization)
technology is used for delivery of genetic and pharmacological material into
cells and tissues and for other nonmechanical processing of bio-matter in
the laboratory, the clinic, and the industrial plant. Despite decades of
study the detailed mechanisms of electroporation and the network of
processes and structures associated with the persistent permeabilized state
remain resistant to characterization.

One proposed mechanism associates persistent membrane permeabilization
following electroporation with the peroxidation of membrane lipids.
Membranes containing oxidized lipid products are leaky, and they remain so
until repaired. Cellular levels of reactive oxygen species are known to
increase after pulsed electric field exposure, and peroxidation products
have been detected in lipid bilayers and cell membranes after
electropermeabilization.

We present a hypothetical scheme for the generation of hydroxyl radicals in
the interior of the lipid bilayer when an electric field is applied, and for
the immediate reaction of these radicals with the phospholipid hydrocarbon
tails located less than 1 nm away. Evidence from experiment and from
molecular simulations supports the plausibility of this proposed mechanism.

 

 

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